Composite

Part:BBa_M36246:Design

Designed by: Bianca Kapoor, Sally Winkler   Group: Stanford BIOE44 - S11   (2011-12-10)


Cadmium Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1905


Design Notes

During design, we used a medium strength promoter to ensure the promoter did not over work the cell.


Source

Watson N., et al, (1992) Identification of Elements Involve in Transcriptional Regulation of the Escherichia coli cad Operon by External pH, Journal of Bacteriology, Vol 174, p. 530-540.

biofab.org

Keselr, I., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A., Mackie A., Paulsen, I., Gunsalus., and Karp, P., (2010), EcoCyc: a comprehensive database of Escherichia coli biology.

Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome. NCBI Reference Sequence: NC_000913.2.

References